ECM® 2001+是ECM2001的升級產品，屬于一臺多功能的細胞電融合和電穿孔儀器。ECM® 2001+能夠快速高效制備雜交瘤細胞和進行核移植操作。獨特的交流正弦波使細胞在雙向電泳作用下排列到一起，然后在微秒級時間內轉換成直流方波使其融合，融合后又一個短暫的交流脈沖穩定雜合細胞的融合狀態，大大提高了細胞融合的效率。相對于ECM2001，交流頻率可調節范圍更大，使電融合效率更高。

ECM® 2001單獨發生直流方波脈沖，可作為電穿孔系統，結合多種專業電極，適用于各種要求的電穿孔實驗，廣泛用于離體和活體電穿孔。

特點：

·         AC 頻率可調節  0.2 – 2.0 MHz

·         直流方波脈沖可單獨作為電穿孔系統

·         電壓調節范圍在5 V 到 3000 V

·         電壓調節分辨率更高

·         一個程序可設置多個AC步驟

·         低電阻下也能操作

·         7寸觸摸屏操作，界面直觀方便

多功能
兼有細胞電融合和電穿孔功能，滿足從簡單轉染到細胞融合一系列實驗操作。
靈活性
擁有較寬的電壓和脈沖時間范圍，可根據實驗方案調整所有脈沖參數，可在8Ω低阻抗下操作；融合過程在顯微鏡下可視操作，提高細胞融合精度。
快速高效 
通過交流非正弦波和直流方波電脈沖的交替作用，可以簡單而快速地完成細胞排列、融合、融合后處理全過程，僅需幾秒鐘。
**性能
電弧淬滅功能，降低電弧引起的損害；短路保護，避免脈沖發生器遇到短路時被損壞。
兼容性
除35mm培養皿電極之外的其他所有BTX電極， 可配2ml和9ml電融合池，提高效率。Enhancer3000監測系統，腳控開關
監控儀選配
Enhancer 3000可以允許用戶監控和記錄主要的電流參數，利用選配的通訊模塊，可以把數據下載到電腦上或在打印機上打印出來。 

應用 

動物細胞融合

核轉移

胚胎操作

雜交瘤生成

植物原生質體融合

活體/離體/卵內基因或drug導入

動物細胞或組織的轉染

干細胞生成

部分原核生物（bacteria）、酵母的轉化              

已發表文獻：

Kim, GA, et al. (2017). Generation of CMAHKO/GTKO/shTNFRI-Fc/HO-1 quadruple gene modified pigs. Transgenic Res. 2017 Aug;26(4): 435-445. doi: 10.1007/s11248-017-0021-6. Epub 2017 May 28.

Sper RB, et al. Generation of a Stable Transgenic Swine Model Expressing a Porcine Histone 2B-eGFP Fusion Protein for Cell Tracking and Chromosome Dynamics Studies. PLoS One. 2017;12(1): e0169242.

Xie Z, et al. Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts. Sci Rep. 2017 Jun;7: 3036. doi: 10.1038/s41598-017-02785-y.

An L, et al. Efficient Generation of FVII Gene Knockout Mice using CRISPR/Cas9 Nuclease and Truncated Guided RNAs. Sci Rep. 2016;6: 25199. doi;10.1038/srep25199.

Bakhshi PK, Bain J, Gul MO, Stride E, Edirisinghe M, Staniland SS. Manufacturing Man‐Made Magnetosomes: High‐Throughput In Situ Synthesis of Biomimetic Magnetite Loaded Nanovesicles. Macromol Biosci. 2016;16: 1555-1561.

Huan, YJ, et al. Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Journal of Reproduction and Development. J Reprod Dec. 2016 Feb;62(1): 71-77. 

Jeong, YH, et al. Knock-in fibroblasts and transgenic blastocysts for expression of human FGF2 in the bovine β-casein gene locus using CRISPR/Cas9 nuclease-mediated homologous recombination.Zygote. 2016;24(3): 442-456.

Lu D, et al. Large-Scale Production of Functional Human Lysozyme from Marker-Free Transgenic Cloned Cows. Sci Rep. 2016;6: 22947. doi: 10.1038/srep22947.

Sun, Y, et al. Deletion of a Yci1 domain protein of Candida albicans allows homothallic mating in MTL heterozygous cells. mBio. 2016 Apr;7(2): e00465-16.

Natalie J, et al. H7N9 influenza virus neutralizing antibodies that possess few somatic mutations. J Clin Invest. 2016 Apr;126(4): 1482-1494.

Wang K, et al. Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-Directed Repair. Mol TherNucleic Acids, 2016 Nov;5(11): e396.

Mangan, PR, et al. Dual inhibition of interleukin-23 and interleukin-17 offers superior efficacy in mouse models of autoimmunity. J Pharmacol Exp Ther. 2015 Aug;354(2), 152-165.

Ruiz N, et al. Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation. PLoS One. 2015 Jun; 10(6): e0130008.

Wang, K, et al. Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system. Sci Rep. 2015 Nov;13(5): 16623.

Wang Y, et al. MiR-499 Enhances the Cisplatin Sensitivity of Esophageal Carcinoma Cell Lines by Targeting DNA Polymerase β. Cell Physiol Biochem. 2015;36: 1587-1596.

Wu H, et al. TALE Nickase-Mediated SP110 Knockin Endows Cattle with Increased Resistance to Tuberculosis. Proc Natl Acad Sci USA. 2015 Mar;112(13): E1530-1539.

Zhang XH, Lian XD, Dai ZX, Zheng HY, Chen X, Zheng YT. α3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2015 Sep;199(6) :2030-2042.

Lai CW, et al. FTSJ2, a Heat Shock-Inducible Mitochondrial Protein, Suppresses Cell Invasion and Migration. PLoS One. 2014 Mar; 9(3): e90818.

Zhang Y, Luo W, Wang Y, Liu Y, and Zheng, L. Purified dendritic cell-tumor fusion hybrids supplemented with non-adherent dendritic cells fraction are superior activators of antitumor immunity. PLoS One, 2014;9(1), e86772.

Liu X, et al. Zinc-Finger Nickase-Mediated Insertion of the Lysostaphin Gene into the Beta-Casein Locus in Cloned Cows. Nat Comm. 2013;4: 2565.

Tan, C, et al. Impact of anti-CD25 monoclonal antibody on dendritic cell-tumor fusion vaccine efficacy in a murine melanoma model. J Transl Med. 2013 Jun;11: 148.

Wang Y, et al. Mir-655 Up-Regulation Suppresses Cell Invasion by Targeting Pituitary Tumor-Transforming Gene-1 in Esophageal Squamous Cell Carcinoma. J Transl Med. 2013;11: 301. 

Clow A, Gaynor P, Oback, B. A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusion. Biomed Microdevices. 2010 Oct;12(5): 777-786.